rt-4 cells  (CLS Cell Lines Service GmbH)

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation, Control

Journal: Cells

Article Title: Artesunate Inhibits Metastatic Potential in Cisplatin-Resistant Bladder Cancer Cells by Altering Integrins.

doi: 10.3390/cells14080570

Figure Lengend Snippet: Figure 1. Tumor cell adhesion to collagen. Adhesion of parental and cisplatin-resistant BCa cells, RT4 (a), RT112 (b), T24 (c), and TCCSup (d) to collagen after exposure to ART. Tumor cells were pre-treated with ART [2.5 or 10 µM] for 48 (RT112, T24, and TCCSup cells) or 72 h (RT4 cells). Adhesion was evaluated after 30 min incubation. Untreated cells served as controls, set to 100%. Pictures under bars represent adhered BCa cells to collagen. Error bars indicate standard deviation (SD). Significant difference to untreated control: * = p ≤0.05, ** = p ≤0.01, *** = p ≤0.001. n = 4.

Article Snippet: Cisplatin-sensitive (parental) BCa cell lines RT4 (grade 1), RT112 (grade 2/3), T24 (grade 3), and TCCSup (grade 4) were purchased from DSMZ.

Techniques: Incubation, Standard Deviation, Control

Journal: Cells

Article Title: Artesunate Inhibits Metastatic Potential in Cisplatin-Resistant Bladder Cancer Cells by Altering Integrins.

doi: 10.3390/cells14080570

Figure Lengend Snippet: Figure 2. Tumor cell adhesion to vascular endothelial cells. Adhesion of parental and cisplatin- resistant BCa cells, RT4 (a), RT112 (b), T24 (c), and TCCSup (d) to HUVECs after treatment with ART [10 µM] for 48 (RT112, T24, and TCCSup cells) or 72 h (RT4 cells). Adhesion was investigated 2 h after incubation. Untreated cells served as controls, set to 100%. Pictures under the bars represent adhered tumor cells. Error bars indicate standard deviation (SD). Significant difference to untreated control: ** = p ≤0.01, *** = p ≤0.001. n = 4.

Article Snippet: Cisplatin-sensitive (parental) BCa cell lines RT4 (grade 1), RT112 (grade 2/3), T24 (grade 3), and TCCSup (grade 4) were purchased from DSMZ.

Techniques: Incubation, Standard Deviation, Control

Journal: Cells

Article Title: Artesunate Inhibits Metastatic Potential in Cisplatin-Resistant Bladder Cancer Cells by Altering Integrins.

doi: 10.3390/cells14080570

Figure Lengend Snippet: Figure 3. Tumor cell chemotaxis, migration, and invasion after ART application. Representative chemotaxis, migration, and invasion pictures of parental (par) and cisplatin-resistant (res) RT4 (a), RT112 (a), T24 (b), and TCCSup (b) cells after treatment with ART. Analysis of chemotaxis, migration, and invasion of RT4 (c), RT112 (d), T24 (e), and TCCSup (f) cell lines pre-treated with ART [10 µM] for 48 (RT112, T24, and TCCSup cells) or 72 h (RT4 cells) evaluated after 24 h incubation in the transwell system, compared to untreated controls. Relative fluorescence (RFU) of untreated controls was set to 100%. Error bars indicate standard deviation (SD). Significant difference to untreated control: * = p ≤0.05, ** = p ≤0.01, *** = p ≤0.001. n = 5.

Article Snippet: Cisplatin-sensitive (parental) BCa cell lines RT4 (grade 1), RT112 (grade 2/3), T24 (grade 3), and TCCSup (grade 4) were purchased from DSMZ.

Techniques: Chemotaxis Assay, Migration, Incubation, Fluorescence, Standard Deviation, Control